العينات الصلبة

1

Weigh 50g solid sample and add 450mL 0.1% peptone water or MRD to the sample

2

It is recommended to use a stomacher bag with filter to eliminate risks of carry over of tiny pieces of foodstuffs into the surface of the medium

3

Homogenize this mixed sample by a blender

4

Open aluminum bag, and take out a set of 4 plates

5

Write the appropriate information on the memorandum section

6

Take off the cap of the plate

7

Pipette 1 ml of homogenized specimen ( to be further diluted if necessary) in the middle of dry sheet of Compact Dry

8

Specimen diffuses automatically and evenly into all over the sheet ( total medium of 20 cm² ) to transform it into gel within seconds

9

Turn over the plate capped

10

Put in an incubator

Count obtained colonies