Solid sample
1
Weigh 50g solid sample and add 450mL 0.1% peptone water or MRD to the sample
2
It is recommended to use a stomacher bag with filter to eliminate risks of carry over of tiny pieces of foodstuffs into the surface of the medium
3
Homogenize this mixed sample by a blender
4
Open aluminum bag, and take out a set of 4 plates
5
Write the appropriate information on the memorandum section
6
Take off the cap of the plate
7
Pipette 1 ml of homogenized specimen ( to be further diluted if necessary) in the middle of dry sheet of Compact Dry
8
Specimen diffuses automatically and evenly into all over the sheet ( total medium of 20 cm2 ) to transform it into gel within seconds
9
Turn over the plate capped
10
Put in an incubator
Count obtained colonies
